Inversion of intron 1 of the factor VIII gene for direct molecular diagnosis of hemophilia A.

a significant long-term decrease of MC was documented and AI probably postponed hematologic relapse. This allowed us to perform a second transplantation in both patients.9 Secondary GVHD grade I-III was seen in 5/14 follow-ups and was fatal in one patient. We confirmed that patients with increasing MC have a significantly higher risk of hematologic relapse.1 Continuous CC together with trMC usually proved to be a good prognostic factor, but in our experience had limited value in predicting extramedullary relapse. Detection of HC is a simple, reliable and rapid method and when performed frequently, allows us to identify patients indicated for AI. A graft-versus-leukemia effect of AI in our small cohort was evident in patients with CML and JMML, was less effective in patients with AML, and was questionable in patients with ALL. We speculate that in patients with acute leukemia AI methods are more effective when initiated early before full leukemia recurrence.